Integrins Are the Necessary Links to Hypertrophic Growth in Cardiomyocytes

Figure 2

Diagram of the collagen overlay model for integrin stimulation in cardiomyocytes with RGD peptide. Cardiomyocytes are plated on laminin-coated plates in media desired for optimal experimental conditions. For integrin stimulation under two-dimensional (2D) conditions (left side of the figure), RGD peptide is added directly in the media to stimulate integrin but with no FAC. For integrin stimulation and FAC formation, (figure on the right), media are removed and replaced with the type I collagen matrix mixed with RGD peptide and allowed to polymerize. Integrins are represented by grey cylinders in the figure.