Repetitive Peroxide Exposure Reveals Pleiotropic Mitogen-Activated Protein Kinase Signaling Mechanisms

Figure 6

Peroxide-mediated subcellular relocalization of EGFR. (a) Subcellular fractionation verification (nuclear, lamin-A: CE3; plasma membrane, Tim23: CE2; cytosol, annexin-V: CE1) and quantitation ((b)-lamin-A, (c)-Tim23, (d)-annexin-V). (e) Peroxide effects (responses R1-R4) on Pyk2 and EGFR subcellular localization Relative expression profiles for Pyk2 (CE1-CE2 quantification (f)-(g)) and EGFR (CE1-CE2 quantification (h)-(i)). Chemical inhibitor-mediated alteration of R4 peroxide-mediated (j) or EGF (k) induced EGFR redistribution from CE2 fraction. EGF (10 ng/mL)-mediated redistribution was compared to non-stimulated (NS) cells.