Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl⁻ Secretion in Mammary Epithelia

Figure 4

((a1) and (a2)) Effect of ATP on iodide efflux in control (a1) and prolactin and dexamethasone-treated (a2) HC-11 cells. (a1) Iodide efflux assays were performed in the absence or presence of 100 μM ATP. (a2) HC-11 cells were pretreated with 1 μg/mL prolactin and dexamethasone (P-D) for 24 h prior to iodide efflux assay. 𝑛≥3. *𝑃<0.05 and ^#𝑃<0.01 versus non-ATP treated groups. ((b1) and (b2)) effect of cAMP cocktails on iodide efflux in control (b1) and prolactin and dexamethasone-treated (b2) HC-11 cells. (b1): Iodide efflux assays were performed in the absence or presence of cAMP cocktails (100 μM cAMP, 10 μM forskolin, and100 μM IBMX). (b2) HC-11 cells were pretreated with 1 μg/mL prolactin and dexamethasone (P-D) for 24 h prior to iodide efflux assay. Data represent mean ± SEM relative to value at starting point. 𝑛≥3. *𝑃<0.05 versus non-cAMP treated groups.