Prolactin and Dexamethasone Regulate Second Messenger-Stimulated Cl− Secretion in Mammary Epithelia
Figure 7
Expression of CFTR mRNA (a), protein (b) and of NKCC1 mRNA (c) in vector-transfected control or PRLR-S-tranfected (rs) HC-11 cells. HC-11 cells were transfected with vector (vtc) or PRLR-S. Transfected cells were treated with or without 1 μg/mL prolactin and dexamethasone (P-D) for 24 h. ((a) and (c)) Total RNA was extracted, and realtime PCR was performed using ribosomal L19 as internal control. (b) Total homogenate of cells were subjected to SDS-PAGE and immunoblotting and probed with a polyclonal CFTR antibody. The blot is representative of 3 experiments. Data represent mean ± SEM relative to vtc, for CFTR and for NKCC1.
