Commitment of Satellite Cells Expressing the Calcium Channel α2δ1 Subunit to the Muscle Lineage
Satellite cells sorted for CD34 and α2δ1. Satellite cells were sorted in four subpopulations and plated in growth medium (20%). Although both subpopulations of cells expressing α2/δ1+ differentiated into myotubes, the α2δ1+/CD34− cells formed longer and thicker myotubes at 7 and 21 days. α2δ1−/CD34− cells transiently produced myotubes, but they did not last past 10 days in culture. α2δ1−/CD34+ cells did not produce myotubes at any time. Images correspond to day 21 in culture. Image sizes are 623 μm 623 μm.