Research Article

Elimination of the Actin-Binding Domain in Kelch-Like 1 Protein Induces T-Type Calcium Channel Modulation Only in the Presence of Action Potential Waveforms

Figure 1

The wild-type KLHL1 actin-binding protein and the truncated mutant ΔKelch both modulate calcium influx through C a v 3.2 upon stimulation with action potentials. (a) Cartoon illustrating the overall structure of KLHL1 and ΔKelch; the amino terminus region (-NH), BTB/POZ, and Kelch domains are represented. (b) Example currents elicited by C a v 3.2 with a square pulse (SQP) from −100 to −25 mV under control conditions (black trace), or in the presence of KLHL1 (red trace) or ΔKelch (green trace). (c) Single-action potential (AP) or 10 Hz action potential waveform (APW) protocols used throughout this work. Examples of the current traces elicited for each condition are shown below, at the 1st and 10th spike during the APW train. (d) Percentage of increase in calcium influx with respect to control.
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