Research Article

Elimination of the Actin-Binding Domain in Kelch-Like 1 Protein Induces T-Type Calcium Channel Modulation Only in the Presence of Action Potential Waveforms

Figure 6

Changes in the repolarization slope in conjunction with an afterhyperpolarization slope synergize to enhance Ca2+ influx by ΔKelch. (a) Protocols with a sloping afterhyperpolarization ramp of 0.75 mV/ms were combined with repolarizing slopes of 70 or 35 mV/ms (blue and orange, resp.), and compared with a waveform with instantaneous repolarization (black). (b) Percentage of increase in calcium influx with the three waveforms with respect to control; the magnitude of the changes induced by the original APWs is shown with dotted lines ( 𝐾 = K L H L 1 ;   Δ 𝐾 = Δ K e l c h ); 𝑃 < 0 . 0 5 .
505346.fig.006a
(a)
505346.fig.006b
(b)