Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis
Figure 3
Reduced susceptibility of CNF1/CNFy-treated cells to trypsin-induced cell detachment. Adherent HeLa cells were treated with CNF1, CNFy, or TcdB for 2 h. Cells were incubated with trypsin for the indicated time and the medium with suspended cells was removed. (a) Adherent cells were documented by phase-contrast microscopy. (b) The number of adherent cells per area was determined in a time-dependent experiment. Results displayed are the mean ± SD of three independent experiments (each experiment cells).