Figure 9: Analysis of calcium channel overlap and GABAB receptors within different areas of the CA1 region of the hippocampus. Regions of interest (ROI’s) were drawn on each image to encompass the entire stratum oriens, the pyramidal cell layer, the stratum radiatum, and the area of highest visible overlap between the fluorophores. The Pearson correlation coefficient was obtained for each ROI and the values for three different rats for all of the regions were averaged. The overlap of CaV1.2 with GABAB was significantly higher in individual cells in the pyramidal cell layer than any other region as well as significantly higher than any overlap seen with CaV1.3 and GABAB receptors (two-way ANOVA followed by Student-Newman-Keuls pairwise comparison, * 𝑃 < 0 . 0 0 1 ).