Angiogenic Signalling Pathways Altered in Gliomas: Selection Mechanisms for More Aggressive Neoplastic Subpopulations with Invasive Phenotype

Figure 4

Intermediate ENU glioma stage showing the expression of Nestin (green) and CD133 (red) by double immunofluorescence. (a–c) Intratumour niches of glioma stem cells positive for Nestin (a) and CD133 (b). (c) Both stem cell markers, Nestin and CD133 are coexpressed in some of these cells. (d–f) Isolated cells in the border of the tumour with a shape similar to astrocytes. Although the majority of these cells are Nestin-CD133 positive, there is a greater density of Nestin+ cells than CD133+ ones. (g–i) Perivascular niche of glioma stem cells displaying colocation of both markers.

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