Journal of Signal Transduction / 2012 / Article / Fig 1

Research Article

Redox Regulation of Nonmuscle Myosin Heavy Chain during Integrin Engagement

Figure 1

Identification of nmMHC as a redox-sensitive protein during cell adhesion. (a) Content of H2O2 in human fibroblasts during adhesion on fibronectin. Hydrogen peroxide production was assayed used DCF-DA. 𝑃 < 0 . 0 0 1 versus time 0. (b) Cell lysates from rounded and spread human fibroblasts were labelled with BIAM and then added to immobilized streptavidin. The pattern of proteins were visualized by a treatment with HRP conjugate streptavidin. (c) Nonredundant (nrNCBI) database was scanned using MASCOT search algorithm. The nonmuscle myosin heavy chain IX of mus musculus (gi/20137006) was identified with a significant score of 115 at 120 ppm mass tolerance. The spectrum shows the matched peaks. (d) The primary structure of nmMHC is shown.

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