Upregulation of Voltage-Gated Calcium Channel Ca_v1.3 in Bovine Somatotropes Treated with Ghrelin

Figure 1

Bovine somatotropes. (a) Histological image (20x) of bovine pituitary stained with haematoxylin-eosin stain. (b) Bovine pituitary cells in primary culture. ((c), (d), and (e)) Bovine somatotropes ICC stained (c) in the absence of specific bGH primary antibody, (d) exposed to the specific bGH primary antibody without the second antibody, and (e) exposed to both specific bGH primary antibody and secondary antibody. (f) Percentage of bGH positive cells found in each Percoll gradient fraction.

(a)

(b)

(c)

(d)

(e)

(f)