Table of Contents
Journal of Toxins
Volume 2013 (2013), Article ID 501876, 10 pages
http://dx.doi.org/10.1155/2013/501876
Research Article

Convulsive and Neurodegenerative Effects in Rats of Some Isolated Toxins from the Tityus bahiensis Scorpion Venom

1Laboratory of Pharmacology, Butantan Institute, Avenida Dr. Vital Brazil 1500, 05503-900 São Paulo, SP, Brazil
2Disease Control Coordinating Agency of São Paulo State Public Health Ministry, 01246-000 São Paulo, SP, Brazil
3Laboratory of Biochemistry and Biophysics, Butantan Institute, 05503-900 São Paulo, SP, Brazil

Received 27 June 2013; Revised 10 September 2013; Accepted 13 September 2013

Academic Editor: Andreimar Martins Soares

Copyright © 2013 Luciene Toshie Takeishi Ossanai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Despite Tityus bahiensis being one of the most dangerous scorpions in Brazil, there are few studies about the effects of its venom, which acts mainly on the central nervous system. Previous studies demonstrated the convulsive ability of this venom. The present work aimed to study the hippocampal effects in rats of some toxins isolated from pool V, which induces a pronounced epileptogenic effect. The pool was separated by reverse-phase HPLC, and the peaks with higher yield (Tb V-1, V-5, V-24, V-27, and V-28) were used in the experiments. Cannulae and electrodes were implanted in the hippocampus of male Wistar rats (240–250 g). The animals were divided into six groups that received intracerebral injection of toxin solution (1 or 2 μg/μL) or Ringer solution (control group), and they were submitted to behavioral, electrographic, and histological analysis. All toxins studied evoked electrographic and behavioral epileptic-like activity to different degrees. Moreover, the toxins V-1, V-24, and V-28 caused significant neuronal loss in CA4 ipsi- and contralateral hippocampal areas. These results suggest that toxins from T. bahiensis scorpion, when injected into the hippocampus, are able to act directly on the central nervous system inducing convulsive and neurodegenerative effects.