Convulsive and Neurodegenerative Effects in Rats of Some Isolated Toxins from the Tityus bahiensis Scorpion Venom
Figure 1
(a) Sephadex G-50 M chromatography of the Tityus bahiensis scorpion venom after extraction with 0.05 M ammonium bicarbonate buffer, pH 8.0, eluted with the same buffer. The eluate was monitored at 280 nm, and 1.5-mL fractions were collected. (b) Analytical HPLC profile of the G-50 active peak on a C18 analytical column eluted with trifluoroacetic acid (0.1%) (solvent) and acetonitrile (90%) (solvent) and a solvent gradient developed from 10–70% within 30 minutes and monitored at 214 nm. Arrows indicate the used toxins.
