Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa

Table 6

Average values of seminal motility parameters, prooxidant/antioxidant markers, and chemical elements in bovine spermatozoa quality groups (Mean ± SEM) and Bonferroni multiple comparison test results.

	Ex (𝑛=11)	Go (𝑛=12)	Mo (𝑛=7)
MOT [%]	93.75±4.50	85.63±3.88^∗a	63.20±3.99^∗∗∗b;∗∗∗c
PROG [%]	90.67±4.57	81.75±1.33^∗a	59.33±6.01^∗∗∗b;∗∗∗c
SOD [U/mg protein]	0.78±0.07	0.65±0.06	0.40±0.07^∗∗b;∗c
CAT [U/mg of protein]	2.37±0.24	2.05±0.23	1.65±0.25^∗b
GSH [mg/mg of protein]	0.02±0.006	0.01±0.007	0.005±0.001^∗b
ALB [mg/mg of protein]	0.13±0.04	0.09±0.005	0.06±0.005^∗b
MDA [𝜇M]	8.74±1.17	14.10±1.15^∗a	16.46±1.36^∗∗b;∗c
Na [mg/dL]	129.60±4.76	114.35±4.33	126.93±4.87
K [mg/dL]	18.10±2.90	23.95±3.13	30.79±3.45^∗∗b;∗c
Fe [mg/dL]	2.39±0.86	2.09±0.61	1.51±0.21^∗b
Cu [mg/dL]	1.37±0.12	1.02±0.10	0.80±0.09^∗b
Mg [mg/dL]	2.65±0.50	1.99±0.40	1.49±0.27^∗b
Zn [mg/dL]	15.07±2.08	10.76±1.13^∗a	7.63±0.926^∗∗b

^aEx versus Go; ^bEx versus Mo; ^cGo versus Mo. *P < 0.05; **P < 0.01; ***P < 0.001. Quality groups are based on the motility values: excellent quality (>90% motile), good quality (80–89% motile), and moderate quality (<79% motile). MOT: motility; PROG: progressive motility; SOD: superoxide dismutase; CAT: catalase; ALB: albumin; GSH: glutathione; MDA: malondialdehyde; Na: Sodium; K: potassium; Fe: iron; Cu: copper, Mg: magnesium; Zn: zinc.