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Journal of Veterinary Medicine
Volume 2013, Article ID 549058, 8 pages
Research Article

S100β Levels in CSF of Nonambulatory Dogs with Intervertebral Disk Disease Treated with Electroacupuncture

1Department of Surgery, School of Veterinary Medicine and Animal Science, The University of São Paulo, 05508-270 São Paulo, SP, Brazil
2Center of Mathematics, Computation and Cognition, The Federal University of ABC, 09210-170 Santo André, SA, Brazil
3Department of Neurology, School of Medicine, The University of São Paulo, 01246-903 São Paulo, SP, Brazil

Received 28 September 2013; Revised 26 November 2013; Accepted 27 November 2013

Academic Editor: Antonio Ortega-Pacheco

Copyright © 2013 Ayne Murata Hayashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the study was to investigate S100β levels in the cerebrospinal fluid of nonambulatory dogs with intervertebral disk disease treated with electroacupuncture: 10 dogs with thoracolumbar disk extrusion graded 3 to 5 (EA group) and 7 dogs without neurologic dysfunction (control group). All dogs regained ambulation. S100β was detected by Western blot analysis where EA group dogs were evaluated at two time points (M1 = before EA and M2 = when the dogs return ambulation) and at one time point from control group. In EA group dogs M1-S100β levels were significantly higher than in control group. EA group dogs were divided into subgroups A ( —early motor recovery; days) and B ( —late motor recovery; days). M1-S100β levels were similar between subgroups A and B. However, M2-S100β levels were significantly higher in subgroup B than in subgroup A. An elevated S100β levels were observed in dogs with late motor recovery. S100β may be associated with neuroplasticity following spinal cord injuries with intervertebral disk extrusion. Further studies with larger numbers of subjects and control group with affected dogs are necessary to investigate the relationship between neurotrophic factors and electroacupuncture stimulation.