Effects of Experimental Sarcocystis neurona-Induced Infection on Immunity in an Equine Model

Figure 7

Effects of PMA/I and merozoite stimulation on peripheral leukocyte proliferation as detected using CFSE. Lymphocytes from control and S. neurona-infect horses were incubated with CFSE and cultured with (a) PMA/I or (b) unstimulated for 72 hrs at 37°C, 5% CO₂. Cells were washed and stained for leukocyte markers, and proliferation based on number of divisions was determined, (c) PMA/I stimulated, and (d) unstimulated.

(a)

(b)

(c)

(d)