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Journal of Veterinary Medicine
Volume 2014, Article ID 325451, 6 pages
Research Article

Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation

1Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden
2Department of Anatomy, Physiology and Biochemistry, SLU, 75007 Uppsala, Sweden

Received 10 June 2014; Accepted 25 July 2014; Published 20 August 2014

Academic Editor: Ronaldo Gonçalves Morato

Copyright © 2014 J. M. Morrell and A. Johannisson. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions. The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (), with significant differences between individuals (). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressive motility was seen for SLC + homologous SP () and for SLC + heterologous SP (). Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused a significant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, ; heterologous SP, ). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk of increased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.