Tamoxifen Promotes Axonal Preservation and Gait Locomotion Recovery after Spinal Cord Injury in Cats

Figure 4

Myelin sheet of T13-L1 ventral axons is illustrated using Toluidine Blue staining. ((a), (f), and (k)) Axons in coronal cuts from an intact cat, ((b), (g), and (l)) ipsilateral axons from an injured cat without tamoxifen, ((c), (h), and (m)) ipsilateral axons from an injured cat with tamoxifen, ((d), (i), and (n)) contralateral axons in a cat without tamoxifen, and ((e), (j), and (o)) contralateral axons in a cat with tamoxifen. Graphs: ordinates exhibit the myelin thickness percentage from ventral zone axons; abscise different studied groups. (p) Myelin axon thickness valued at 200 μm rostral from the injury, (q) axon myelin thickness valued in the injury site, (r) axon myelin thickness valued 200 μm caudal from the injury situ, ; scale bar: 5 μm. Schematic diagram illustrates in site the rostral and caudal zones to value myelin thickness. The black horizontal line over the bars indicates a statistical significant difference between referred groups.