Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells

Table 1

Antibodies used in this study.

Target molecule	Abbreviation	Species isotype	Label	Company	Clone	Dilution
Phosphorylated Human β-Catenin (pS45) Peptide	β-catenin (pS45)	Mouse (BALB/c) IgG1, κ	Alexa Fluor 647	BD Biosciences	K63-363	1 : 50
Mouse β-Catenin aa. 571-781	β-catenin	Mouse IgG1	Alexa Fluor 488	BD Biosciences	14/Beta-Catenin	1 : 200
Phosphorylated Human Akt1 (pT308) Peptide	Akt (pT308)	Mouse IgG1, κ	Phycoerythrin	BD Biosciences	J1-223.371	1 : 50
Phosphorylated Human Akt1 (pS473) Peptide	Akt (pS473)	Mouse (BALB/c) IgG1, κ	Alexa Fluor 647	BD Biosciences	M89-61	1 : 50
Phosphorylated Human PDGFRβ (pY1009) Peptide	CD140b	Mouse IgG2b, κ	Phycoerythrin	BD Biosciences	J25-602	1 : 50
Phosphorylated Human Stat1 (pY701) Peptide	Stat1	Mouse IgG2a	Alexa Fluor 488	BD Biosciences	4a	1 : 50
Phosphorylated Human Stat3 (pY705) Peptide	Stat3	Mouse IgG2a, κ	Phycoerythrin	BD Biosciences	4/P-STAT3	1 : 50
Phosphorylated Human Stat4 (pY693) Peptide	Stat4	Mouse IgG2b, κ	Alexa Fluor 647	BD Biosciences	38/p-Stat4	1 : 50
Phosphorylated Human Stat5 (pY694) Peptide	Stat5	Mouse IgG1, κ	Alexa Fluor 488	BD Biosciences	47/Stat5 (pY694)	1 : 50
Phosphorylated Human Stat6 (pY641) Peptide	Stat6	Mouse IgG1, κ	Phycoerythrin	BD Biosciences	J71-773.58.11	1 : 50
Phosphorylated Human p38 MAPK (pT180/pY182) Peptide	p38 MAPK	Mouse IgG1, κ	Phycoerythrin	BD Biosciences	36/p38 (pT180/pY182)	1 : 50
Phosphorylated Human Smad2 (pS465/pS467)/Smad3 (pS423/pS425) Peptide	Smad2/3	Mouse IgG1, κ	Alexa Fluor 647	BD Biosciences	O72-670	1 : 200
Phosphorylated Rat ERK1 (pT202/pY204) Peptide	ERK1/2	Mouse IgG1	Alexa Fluor 488	BD Biosciences	20A	1 : 50