Suspected Lonely Mouse Syndrome as a Cage Effect in a Drug Safety Study

Table 1

Experimental design displaying the number of daily observations and harvest schedule for the single dose (Trial 1) and repeat dose (Trial 2) studies.

	Trial day
	Single dose: Trial 1
Number of mice	Days 1	2	3	4	5
Per dose 16♂, 16♀	32
per harvest		16		16
Charts AM		32	16	16
Charts PM	32	16	16	16
	Repeat dose: Trial 2
Number of mice	Days 1	2 to 3	4	5	6	7	8	9 to 11	12
Per dose 16♂, 16♀	32		32				16
Per harvest					16				16
Charts AM		32	32	32	32	16	16	16
Charts PM	32	32	32	32	16	16	16	16

Weight, hematology, and clinical chemistry; weight, hematology, clinical chemistry, coagulation panel, organ weight, and histopathology.