Table of Contents
Journal of Waste Management
Volume 2013, Article ID 630209, 7 pages
Research Article

Removal of Olive Mill Wastewater Phenolics with the Use of a Polyphenol Oxidase Homogenate from Potato Peel Waste

1Laboratory of Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M.A.I.Ch.), P.O. Box 85, 73100 Chania, Greece
2School of Environment, University of the Aegean, Mitr. Ioakim Street, Lemnos, 81400 Myrina, Greece

Received 17 September 2013; Accepted 1 November 2013

Academic Editor: Milva Pepi

Copyright © 2013 Florin Daniel Demian and Dimitris P. Makris. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Olive mill wastewater (OMW) originating from a two-phase olive oil producing plant was treated with a crude polyphenol oxidase (PPO) homogenate, prepared from potato waste peels. The treatments carried out were based on a 23-full-factorial, central composite design (CCD) in order to identify optimal operational conditions with regard to polyethylene glycol (PEG) concentration, pH, and treatment duration. The treatment performance was assessed by estimating the % reduction in total polyphenol (TP) concentration. The model obtained produced a satisfactory fitting of the data ( , ). The utilisation of the predictive model enabled the theoretical calculation of the optimal set of conditions, which were ,  h, and [PEG] = 900 mg L−1. Under these conditions, the optimal theoretical % removal calculated was . Examination of the treated samples with high-performance liquid chromatography (HPLC) showed that the potato homogenate afforded changes in the polyphenolic profile. Based on the experimental evidence, oxidation pathways were proposed.