Laser Chemistry

Laser Chemistry / 1999 / Article
Special Issue

Proceedings of the Eighth Conference on Time-Resolved Vibrational Spectroscopy

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Volume 19 |Article ID 054345 |

Olaf Weidlich, Christoph Rödig, Friedrich Siebert, "Time-Resolved Step-Scan Ftir Investigations on the M1→M2 Transition in the Light-Driven Proton Pump Bacteriorhodopsin", Laser Chemistry, vol. 19, Article ID 054345, 7 pages, 1999.

Time-Resolved Step-Scan Ftir Investigations on the M1→M2 Transition in the Light-Driven Proton Pump Bacteriorhodopsin

Received06 Apr 1997


Using time-resolved step-scan FTIR spectroscopy, it has become possible to identify two different M states in the 5 to 300 μs time range, which clearly differ from the MN state. The identification has become possible by measuring a pure BR→KL difference spectrum at 100 ns, which can be used to correct for the contributions of this intermediate in the later difference spectra. The subtraction of the later corrected difference spectra thus represent L→M1, M2 difference spectra with varying relative amount of the two M states. The comparison of these spectra clearly reveals differences in the amide-II spectral range, and possibly also in the amide-I range. From these observations it can be concluded that the two M states do not differ in the chromophore structure but in the protein structure.

Copyright © 1999 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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