Table of Contents
Laser Chemistry
Volume 2009, Article ID 474858, 14 pages
http://dx.doi.org/10.1155/2009/474858
Research Article

Development, Characterization, and Application of a Versatile Single Particle Detection Apparatus for Time-Integrated and Time-Resolved Fluorescence Measurements—Part II: Experimental Evaluation

Department of Chemistry, University of Florida, Gainesville, FL 32611, USA

Received 10 February 2009; Accepted 13 April 2009

Academic Editor: Savas K. Georgiou

Copyright © 2009 Xihong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. C. Hinds, Aerosol Technology: Properties, Behavior and Measurement of Airborne Particles, John Wiley & Sons, New York, NY, USA, 2nd edition, 1999.
  2. P. Liu, P. J. Ziemann, D. B. Kittelson, and P. H. McMurry, “Generating particle beams of controlled dimensions and divergence—I: theory of particle motion in aerodynamic lenses and nozzle expansions,” Aerosol Science and Technology, vol. 22, no. 3, pp. 293–313, 1995. View at Google Scholar
  3. P. Liu, P. J. Ziemann, D. B. Kittelson, and P. H. McMurry, “Generating particle beams of controlled dimensions and divergence—II: experimental evaluation of particle motion in aerodynamic lenses and nozzle expansions,” Aerosol Science and Technology, vol. 22, no. 3, pp. 314–324, 1995. View at Google Scholar
  4. B. E. Dahneke and Y. S. Cheng, “Properties of continuum source particle beams. I. Calculation methods and results,” Journal of Aerosol Science, vol. 10, no. 3, pp. 257–274, 1979. View at Google Scholar
  5. Y. S. Cheng and B. E. Dahneke, “Properties of continuum source particle beams. II. Beams generated in capillary expansions,” Journal of Aerosol Science, vol. 10, no. 4, pp. 363–368, 1979. View at Google Scholar
  6. T. J. Estes, V. L. Vilker, and S. K. Friedlander, “Characteristics of a capillary-generated particle beam,” Journal of Colloid and Interface Science, vol. 93, no. 1, pp. 84–94, 1983. View at Google Scholar
  7. R. V. Mallina, A. S. Wexler, and M. V. Johnston, “High-speed particle beam generation: simple focusing mechanisms,” Journal of Aerosol Science, vol. 30, no. 6, pp. 719–738, 1999. View at Publisher · View at Google Scholar
  8. J. Schreiner, U. Schild, C. Voigt, and K. Mauersberger, “Focusing of aerosols into a particle beam at pressures from 10 to 150 Torr,” Aerosol Science and Technology, vol. 31, no. 5, pp. 373–382, 1999. View at Publisher · View at Google Scholar
  9. X. Zhang, K. A. Smith, D. R. Worsnop, J. Jimenez, J. T. Jayne, and C. E. Kolb, “A numerical characterization of particle beam collimation by an aerodynamic lens-nozzle system—part I: an individual lens or nozzle,” Aerosol Science and Technology, vol. 36, no. 5, pp. 617–631, 2002. View at Publisher · View at Google Scholar
  10. X. Zhang, K. A. Smith, D. R. Worsnop et al., “Numerical characterization of particle beam collimation—part II: integrated aerodynamic-lens-nozzle system,” Aerosol Science and Technology, vol. 38, no. 6, pp. 619–638, 2004. View at Publisher · View at Google Scholar
  11. J. Marijnissen, B. Scarlett, and P. Verheijen, “Proposed on-line aerosol analysis combining size determination, laser-induced fragmentation and time-of-flight mass spectroscopy,” Journal of Aerosol Science, vol. 19, no. 7, pp. 1307–1310, 1988. View at Google Scholar
  12. M. V. Johnston and A. S. Wexler, “Mass spectrometry of individual aerosol particles,” Analytical Chemistry, vol. 67, pp. 721A–726A, 1995. View at Google Scholar
  13. M. Weiss, P. J. T. Verheijen, J. C. M. Marijnissen, and B. Scarlett, “On the performance of an on-line time-of-flight mass spectrometer for aerosols,” Journal of Aerosol Science, vol. 28, no. 1, pp. 159–171, 1997. View at Publisher · View at Google Scholar
  14. C. A. Noble and K. A. Prather, “Real-time measurement of correlated size and composition profiles of individual atmospheric aerosol particles,” Environmental Science and Technology, vol. 30, no. 9, pp. 2667–2680, 1996. View at Publisher · View at Google Scholar
  15. C. A. Noble and K. A. Prather, “Aerosol time-of-flight mass spectrometry,” in Analytical Chemistry of Aerosols, K. R. Spurny, Ed., pp. 353–376, Lewis, Boca Raton, Fla, USA, 1999. View at Google Scholar
  16. D. T. Suess and K. A. Prather, “Mass spectrometry of aerosols,” Chemical Reviews, vol. 99, no. 10, pp. 3007–3035, 1999. View at Google Scholar
  17. M. V. Johnston, “Sampling and analysis of individual particles by aerosol mass spectrometry,” Journal of Mass Spectrometry, vol. 35, no. 5, pp. 585–595, 2000. View at Publisher · View at Google Scholar
  18. M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, B. Scarlett, B. L. M. Van Baar, and Ch. E. Kientz, “Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry,” Rapid Communications in Mass Spectrometry, vol. 14, no. 10, pp. 829–833, 2000. View at Publisher · View at Google Scholar
  19. D. B. Kane, B. Oktem, and M. V. Johnston, “Nanoparticle detection by aerosol mass spectrometry,” Aerosol Science and Technology, vol. 34, no. 6, pp. 520–527, 2001. View at Google Scholar
  20. S. N. Jackson, S. Mishra, and K. K. Murray, “On-line laser desorption/ionization mass spectrometry of matrix-coated aerosols,” Rapid Communications in Mass Spectrometry, vol. 18, no. 18, pp. 2041–2045, 2004. View at Publisher · View at Google Scholar · View at PubMed
  21. N. Erdmann, A. Dell'Acqua, P. Cavalli et al., “Instrument characterization and first application of the single particle analysis and sizing system (SPASS) for atmospheric aerosols,” Aerosol Science and Technology, vol. 39, no. 5, pp. 377–393, 2005. View at Publisher · View at Google Scholar
  22. D. G. Nash, T. Baer, and M. V. Johnston, “Aerosol mass spectrometry: an introductory review,” International Journal of Mass Spectrometry, vol. 258, no. 1–3, pp. 2–12, 2006. View at Publisher · View at Google Scholar
  23. M. Tolocka, M. Reinard, D. Lake, J. Ondov, A. Wexler, and M. Johnston, “Characterization of short-term particulate matter events by real-time single particle mass spectrometry,” in Aerosol Science and Technology, vol. 40, no. 10, pp. 873–882, 2006. View at Publisher · View at Google Scholar
  24. M. S. Reinard, K. Adou, J. M. Martini, and M. V. Johnston, “Source characterization and identification by real-time single particle mass spectrometry,” Atmospheric Environment, vol. 41, no. 40, pp. 9397–9409, 2007. View at Publisher · View at Google Scholar
  25. C. A. Zordan, S. Wang, and M. V. Johnston, “Time-resolved chemical composition of individual nanoparticles in urban air,” Environmental Science and Technology, vol. 42, no. 17, pp. 6631–6636, 2008. View at Publisher · View at Google Scholar
  26. M. S. Reinard and M. V. Johnston, “Ion formation mechanism in laser desorption ionization of individual nanoparticles,” Journal of the American Society for Mass Spectrometry, vol. 19, no. 3, pp. 389–399, 2008. View at Publisher · View at Google Scholar · View at PubMed
  27. K. R. Spurny, “On the chemical detection of bioaerosols,” Journal of Aerosol Science, vol. 25, no. 8, pp. 1533–1547, 1994. View at Google Scholar
  28. A. L. van Wuijckhuijse, M. A. Stowers, W. A. Kleefsman, B. L. M. van Baar, Ch. E. Kientz, and J. C. M. Marijnissen, “Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens,” Journal of Aerosol Science, vol. 36, no. 5-6, pp. 677–687, 2005. View at Publisher · View at Google Scholar
  29. I. Kleefsman, M. A. Stowers, P. J. T. Verheijen, A. L. van Wuijckhuijse, Ch. E. Kientz, and J. C. M. Marijnissen, “Bioaerosol analysis by single particle mass spectrometry,” Particle and Particle Systems Characterization, vol. 24, no. 2, pp. 85–90, 2007. View at Publisher · View at Google Scholar
  30. P. P. Hairston, J. Ho, and F. R. Quant, “Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence,” Journal of Aerosol Science, vol. 28, no. 3, pp. 471–482, 1997. View at Publisher · View at Google Scholar
  31. A. P. Snyder, Ed., “Chemical and Biological aerosol detection and identification with field analytical instrumentation,” Field Analytical Chemistry and Technology, vol. 3, pp. 219–326, 1999. View at Google Scholar
  32. S. C. Hill, R. G. Pinnick, S. Niles et al., “Real-time measurement of fluorescence spectra from single airborne biological particles,” Field Analytical Chemistry and Technology, vol. 3, no. 4-5, pp. 221–239, 1999. View at Google Scholar
  33. Y. L. Pan, R. G. Pinnick, S. C. Hill, R. K. Chang et al., “Particle fluorescence spectrometer for real-time single particle measurements of atmospheric organic carbon and biological aerosols,” Environmental Science and Technology, vol. 43, no. 2, pp. 429–434, 2009. View at Google Scholar
  34. M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, Jr., and D. C. Roselle, “Size and fluorescence measurements for field detection of biological aerosols,” Aerosol Science and Technology, vol. 30, no. 2, pp. 174–185, 1999. View at Publisher · View at Google Scholar
  35. V. Sivaprakasam, A. L. Huston, C. Scotto, and J. D. Eversole, “Multiple UV wavelength excitation and fluorescence of bioaerosols,” Optics Express, vol. 12, no. 19, pp. 4457–4466, 2004. View at Publisher · View at Google Scholar
  36. Y.-L. Pan, P. Cobler, S. Rhodes et al., “High-speed, high-sensitivity aerosol fluorescence spectrum detection using a 32-anode photomultiplier tube detector,” Review of Scientific Instruments, vol. 72, no. 3, pp. 1831–1836, 2001. View at Publisher · View at Google Scholar
  37. P. H. Kaye, W. R. Stanley, E. Hirst, E. V. Foot, K. L. Baxter, and S. J. Barrington, “Single particle multichannel bio-aerosol fluorescence sensor,” Optics Express, vol. 13, no. 10, pp. 3583–3593, 2005. View at Publisher · View at Google Scholar
  38. Y.-L. Pan, J. Hartings, R. G. Pinnick, S. C. Hill, J. Halverson, and R. K. Chang, “Single-particle fluorescence spectrometer for ambient aerosols,” Aerosol Science and Technology, vol. 37, no. 8, pp. 628–639, 2003. View at Publisher · View at Google Scholar
  39. K. Davitt, Y.-K. Song, W. R. Patterson, III et al., “290 and 340 nm UV LED arrays for fluorescence detection from single airborne particles,” Optics Express, vol. 13, no. 23, pp. 9548–9555, 2005. View at Publisher · View at Google Scholar
  40. K. Davitt, Y.-K. Song, W. R. Patterson, III et al., “Spectroscopic sorting of aerosols by a compact sensor employing UV LEDs,” Aerosol Science and Technology, vol. 40, no. 12, pp. 1047–1051, 2006. View at Publisher · View at Google Scholar
  41. D. P. Fergenson, M. E. Pitesky, H. J. Tobias et al., “Reagentless detection and classification of individual bioaerosol particles in seconds,” Analytical Chemistry, vol. 76, no. 2, pp. 373–378, 2004. View at Publisher · View at Google Scholar · View at PubMed
  42. M. A. Stowers, A. L. van Wuijckhuijse, J. C. M. Marijnissen, Ch. E. Kientz, and T. Ciach, “Fluorescence preselection of bioaerosol for single-particle mass spectrometry,” Applied Optics, vol. 45, no. 33, pp. 8531–8536, 2006. View at Publisher · View at Google Scholar
  43. X. Wu, N. Omenetto, B. W. Smith, and J. D. Winefordner, “Single particle fluorescence: a simple experimental approach to evaluate coincidence effects,” Applied Spectroscopy, vol. 61, no. 7, pp. 711–718, 2007. View at Publisher · View at Google Scholar · View at PubMed
  44. H. C. Huang, Y.-L. Pan, S. C. Hill, R. G. Pinnick, and R. K. Chang, “Real-time measurement of dual-wavelength laser-induced fluorescence spectra of individual aerosol particles,” Optics Express, vol. 16, no. 21, pp. 16523–16528, 2008. View at Publisher · View at Google Scholar
  45. J. D. Hybl, G. A. Lithgow, and S. G. Buckley, “Laser-induced breakdown spectroscopy detection and classification of biological aerosols,” Applied Spectroscopy, vol. 57, no. 10, pp. 1207–1215, 2003. View at Publisher · View at Google Scholar
  46. P. B. Dixon and D. W. Hahn, “Feasibility of detection and identification of individual bioaerosols using laser-induced breakdown spectroscopy,” Analytical Chemistry, vol. 77, no. 2, pp. 631–638, 2005. View at Publisher · View at Google Scholar · View at PubMed
  47. J. D. Hybl, S. M. Tysk, S. R. Berry, and M. P. Jordan, “Laser-induced fluorescence-cued, laser-induced breakdown spectroscopy biological-agent detection,” Applied Optics, vol. 45, no. 34, pp. 8806–8814, 2006. View at Publisher · View at Google Scholar
  48. X. Wu, N. Omenetto, and J. D. Winefordner, “Development, characterization and application of a versatile single particle detection apparatus for time-integrated and timeresolved fluorescence measurements—part 1: theoretical considerations,” Laser Chemistry. In press. View at Publisher · View at Google Scholar
  49. K. Salt, C. A. Noble, and K. A. Prather, “Aerodynamic particle sizing versus light scattering intensity measurement as methods for real-time particle sizing coupled with time-of-flight mass spectrometry,” Analytical Chemistry, vol. 68, no. 1, pp. 230–234, 1996. View at Google Scholar
  50. G. A. Petrucci, P. B. Farnsworth, P. Cavalli, and N. Omenetto, “A differentially pumped particle inlet for sampling of atmospheric aerosols into a time-of-flight mass spectrometer: optical characterization of the particle beam,” Aerosol Science and Technology, vol. 33, no. 1-2, pp. 105–121, 2000. View at Publisher · View at Google Scholar
  51. J. A. Merten, N. Omenetto, X. Wu, B. W. Smith, and J. D. Winefordner, “Time and wavelength resolved laser-induced amino acid fluorescence of bacteria,” to be submitted to Applied Optics.
  52. Y.-L. Pan, S. C. Hill, J.-P. Wolf, S. Holler, R. K. Chang, and J. R. Bottiger, “Backward-enhanced fluorescence from clusters of microspheres and particles of tryptophan,” Applied Optics, vol. 41, no. 15, pp. 2994–2999, 2002. View at Google Scholar
  53. K. Willeke and P. A. Baron, Aerosol Measurement: Principles, Techniques and Applications, Van Nostrand Reynolds, New York, NY, USA, 1993.
  54. A. R. Holzwarth, “Time-resolved fluorescence spectroscopy,” in Biochemical Spectroscopy, vol. 246, pp. 334–362, 1995. View at Google Scholar
  55. S. R. Piersma, A. J. W. G. Visser, S. De Vries, and J. A. Duine, “Optical spectroscopy of nicotinoprotein alcohol dehydrogenase from Amycolatopsis methanolica: a comparison with horse liver alcohol dehydrogenase and UDP-galactose epimerase,” Biochemistry, vol. 37, no. 9, pp. 3068–3077, 1998. View at Publisher · View at Google Scholar · View at PubMed
  56. J. M. Beechem and L. Brand, “Time-resolved fluorescence of proteins,” Annual Review of Biochemistry, vol. 54, pp. 43–71, 1985. View at Google Scholar
  57. A. Thomas, D. Sands, D. Baum, L. To, and G. O. Rubel, “Emission wavelength dependence of fluorescence lifetimes of bacteriological spores and pollens,” Applied Optics, vol. 45, no. 25, pp. 6634–6639, 2006. View at Publisher · View at Google Scholar