PKCδ Regulates Translation Initiation through PKR and eIF2α in Response to Retinoic Acid in Acute Myeloid Leukemia Cells
Figure 1
ATRA- and ATO-mediated effects in APL cells. APL (NB4) cells were kept in growth medium with ATRA (1 M) or without ATRA for the indicated time periods. The cells were stained with monoclonal anti-CD11b (a) or anti-CD11c ((b) and (c)) antibodies to detect induction of granulocytic differentiation and analyzed by flow cytometry. (d) ATO inhibits growth of NB4 cells. NB4 cells were treated with ATO at the indicated concentrations for 48 h and viable cells were counted as described in Section 2. ATO-induced apoptosis was determined by the activation of caspases 9 and 3 as well as cleavage of PARP in NB4 cells (see Supplementary Figure 2 in Supplementary Material available online at doi: 10.1155/2012/482905).
