PKCδ Regulates Translation Initiation through PKR and eIF2α in Response to Retinoic Acid in Acute Myeloid Leukemia Cells
Figure 2
ATRA and ATO induce phosphorylation of eIF2α. (a) NB4 cells were treated with ATRA (0.1 μM or 1 μM) for the indicated time periods or left untreated. Equal amounts of total cell lysate were analyzed by SDS-PAGE and immunoblotted with antiphospho (Ser51) eIF2α or eIF2α antibodies. β-Actin was used as loading control for the Western blots. The panel (below) represents densitometric analysis shows relative expression of p-eIF2α after normalization to total eIF2α and actin levels. (b) ATO (0.4 μM or 1 μM) induces phosphorylation (Ser51) of eIF2α and total eIF2α in a time-dependent manner in NB4 cells. Densitometry analysis (below) represents relative p(Ser51)-eIF2α expression seen in the blots.
