Mycophenolic Acid Overcomes Imatinib and Nilotinib Resistance of Chronic Myeloid Leukemia Cells by Apoptosis or a Senescent-Like Cell Cycle Arrest
Figure 5
MPA induced apoptosis in CD34 CML cells. Primary CD34 cells isolated from blood samples of CML patients responding to imatinib (patient 1) or resistant to imatinib and nilotinib (patient 2 to 4) were grown in the presence of vehicle only, imatinib 1 μM, or MPA 3 μg/mL for 3 days. Patient 2 and 3 overexpressed several tyrosine kinase such as Src-kinase, whereas patient 4 was mutated in Abl kinase domain harbouring the T315I mutation. Annexin-V binding was detected at day 0 and upon treatment at day 3.
