New Quantitative Method to Identify NPM1 Mutations in Acute Myeloid Leukaemia

Table 2

ΔCT obtained for each mutation type with the five different specific primers.

NPM1 mutation	Specific primer
	Primer; A	Primer; B	Primer; C	Primer; D	Primer; P
A	−3	14	18	−2	18
B	−2	−4	1	−2	3
C	−2	−3	−4	−3	3
D	−3	1	7	−3	5
P	4	15	16	13	−3
Negative control (wild type)	No CT obtained

The ΔCT profiles (i.e., the 5 values obtained in one sample) are specific for the NPM1m type.