Metformin Induces Cell Cycle Arrest and Apoptosis in Drug-Resistant Leukemia Cells

Figure 2

Effects of metformin on apoptosis. CEM and 10E₁-CEM cells were exposed to 10 mM metformin for 72 h and the induction of apoptosis was evaluated by (a, and Table 1) flow cytometry of annexin V-PI labeled cells and (b) by fluorescence microscopy of DAPI labeled cells. (c) 10E₁-CEM cells were treated with 10 mM metformin for 96 h before caspase-3 and -7 activation was evaluated by flow cytometry. The numbers in the quadrants indicate the percentages of cells. A representative experiment of three performed. (a, and Table 1).

(a)

(b)

(c)