Metformin Induces Cell Cycle Arrest and Apoptosis in Drug-Resistant Leukemia Cells

Figure 7

Metformin induces mitochondrial perturbations. CEM and 10E₁-CEM cells were exposed to metformin (10 mM) for 48 h and then stained with DiOC₆(3)/PI. The was determined as DiOC₃(6) emitted fluorescence (black: control cells, red: metformin-treated cells). Fluorescence by CEM cells treated with 4-HPR has been shown as positive control (CEM CTRL+). In all cases, differences of mean fluorescence intensity of metformin versus control ( cells analyzed/assay) are statistically significant () for each experiment of the three performed.