Metformin Induces Cell Cycle Arrest and Apoptosis in Drug-Resistant Leukemia Cells
Table 3
Densitometric analysis of cell cycle regulatory proteins.
Control
24 h
48 h
72 h
CEM
Cdc2 p34
1
0.96 ± 0.19
0.93 ± 0.27
0.47 ± 0.12
Cyclin B1
1
2.37 ± 0.59
0.77 ± 0.36
0.33 ± 0.08
Cyclin A
1
3.60 ± 1.25
1.65 ± 0.42
0.73 ± 0.15
Cyclin E
1
0.84 ± 0.11
1.29 ± 0.08
1.02 ± 0.03
10E1-CEM
Cdc2 p34
1
1.37 ± 0.32
0.65 ± 0.18
0.12 ± 0.01
Cyclin B1
1
1.86 ± 0.85
0.67 ± 0.23
0.15 ± 0.03
Cyclin A
1
1.28 ± 0.07
0.61 ± 0.19
0.24 ± 0.04
Cyclin E
1
1.12 ± 0.04
0.67 ± 0.13
1.90 ± 0.30
R5-CEM
Cdc2 p34
1
0.90 ± 0.08
1.31 ± 0.05
2.34 ± 0.91
Cyclin B1
1
1.45 ± 0.24
2.46 ± 0.59
1.77 ± 0.25
Cyclin A
1
1.35 ± 0.13
1.43 ± 0.36
1.25 ± 0.09
Cyclin E
1
1.35 ± 0.15
1.47 ± 0.21
1.53 ± 0.16
Data are expressed as fold changes compared with controls, normalized to actin, and represent mean ± SD of three experiments. , NS (nonsignificant) for metformin versus control at 72 h.
