Table of Contents
Metal-Based Drugs
Volume 4, Issue 4, Pages 207-219
http://dx.doi.org/10.1155/MBD.1997.207

Behaviour of the Antitumor Agent Vanadocene Dichloride in Physiological and Therapeutic Media, Blood Plasma and Human Blood - An EPR Study

Department of General and Inorganic Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice CZ-532 10, Czech Republic

Received 14 April 1997; Accepted 15 May 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

By means of EPR spectroscopy the behaviour of the vanadocene dichloride (I), Cp2VCl2(Cp=η5-C5H5), in various deoxygenated and non-deoxygenated physiological media and therapeutic solution as well as in blood plasma and stabilized human blood was studied. On the basis of measured values of isotropic spectroscopic splitting factor giso and isotropic hyperfine coupling constant Aiso, the vanadocene species, [Cp2V(H2O)Cl]+ (II; giso= 1.985, |Aiso| = 7.68 mT), [Cp2V(H2O)2]2+ (III; giso= 1.983, |Aiso| = 7.92 mT), Cp2V(OH)2 (IV; giso= 1.991, |Aiso| = 6.285 mT), [Cp2VCl(DMSO)]+ (V; giso= 1.985, |Aiso| = 7.69 mT), the vanadyl species [VO(DMSO)5]2+ (VI; giso= 1.964, |Aiso| = 10.78mT) and [VO(H2O)5]2+ (VII; giso= 1.955, |Aiso| = 11.56 mT) have been identified. From the measurements it follows that I does not react in its first coordination sphere with any component of a system used other than water and DMSO, resp. As to water-containing media, its behaviour is fully consistent with that of I in pure aqueous media. It was found the only vanadocene species present after application of the therapeutic solution of I into human blood to be IV not interacting in its first coordination sphere with any blood component.