Identification of Proteins Related to Nickel Homeostasis in Helicobater pylori by Immobilized Metal Affinity Chromatography and Two-Dimensional Gel Electrophoresis

Table 1

Summary of nickel-interacting H. pylori proteins identified by peptide mass fingerprinting.

Protein no.	Protein name	Identified peptides	% Sequence coverage	MOWSE score	MW (kDa)/pI
					Theor.	Obs.
1^*	UreB	21	42	1.595e+10	61.4/5.5	65.5/5.8
2^*	60 kDa chaperonin (HspB)	65	75	1.199e+15	58.2/5.5	59.3/5.3
3	60 kDa chaperonin (HspB)	19	37	9.875e+04	58.2/5.5	56.7/5.5
4	Inosine-1∶57-monophosphate dehydrogenase (GuaB)	24	51	2.559e+09	51.8/7.7	56.2/8.0
5	Fumarase	22	51	1.097e+11	51.0/6.5	52.6/7.4
6	Fumarase	20	45	1.800e+10	51.0/6.5	52.6/7.0
7	Putative aminotransferase	26	55	2.466e+12	42.4/5.8	40.4/6.2
8	Hypothetical protein HP0271 (fragment)	4	12	980	38.6/5.0	21.1/4.8
9	Cinnnamyl-alcohol dehydrogenase ELI3-2 (Cad)	15	36	1.912e+09	38.6/7.0	40.7/7.8
10	Cell binding factor 2	21	48	5.030e+06	34.0/9.3	31.9/9.8
11	Outer membrane protein jhp1472	11	32	6.291e+04	30.2/9.1	26.7/9.3
12	Hypothetical protein jhp0216	15	57	7.869e+05	29.5/9.1	27.4/9.4
13	Hypothetical protein jhp0301	7	24	1.027e+04	28.6/7.8	27.1/8.0
14	Hypothetical protein jhp0301	19	61	2.461e+05	28.6/7.8	27.1/8.1
15	Putative neuraminyllactose-binding hemagglutinin homolog (HpaA)	13	50	2.381e+05	28.3/7.9	27.0/7.2
16	Urease alpha subunit (UreA)	14	68	1.280e+07	26.5/8.5	27.2/9.0
17	Urease alpha subunit (UreA)	4	88	1.846e+11	26.5/8.5	27.2/8.5
18	Putative alkyl hydroperoxide reductase (TsaA)	12	54	1.297e+07	21.9/5.7	19.9/6.2
19	Inorganic pyrophosphatase (PPase)	4	23	575	14.7/5.0	20.7/4.7
20	Non-heme iron containing ferritin (Pfr)	2	23	369	19.3/5.4	14.4/5.2
21	Non-heme rion containing ferretin (Pfr)	8	56	2.190e+07	19.3/5.4	14.4/5.5
22	Hypothetical protein HP0721	13	44	1.692e+04	17.6/8.9	14.4/8.5
23	Neutrophil activating protein (NapA)	6	48	1.570e+05	16.8/5.7	10.2/6.2
24	Hypothetical protein jhp0118	4	32	1.620e+03	16.6/9.3	12.2/9.8
25	Chaperonin groES (HspA)	6	44	146	13.0/6.1	10.2/6.4
26	Hepothetical protein HP0614	13	56	1.557e+05	13.0/7.0	7.7/8.0
27	4-oxalocrotonate tautomerase (DmpI)	10	85	3.420e+05	7.5/6.0	5.5/6.4

^*Confirmed with 2-DE images of H. pylori 26695 (http://www.mpiib-berlin.mpg.de/2D-PAGE/EBP-PAGE/index.html).