Table of Contents
Molecular Biology International
Volume 2010, Article ID 240472, 6 pages
http://dx.doi.org/10.4061/2010/240472
Research Article

Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo

1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
2Central Laboratory, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

Received 23 June 2010; Revised 1 September 2010; Accepted 30 September 2010

Academic Editor: Van Luu-The

Copyright © 2010 Jia Xiu-Hua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. K. U. Schallreuter, S. Kothari, B. Chavan, and J. D. Spencer, “Regulation of melanogenesis-controversies and new concepts,” Experimental Dermatology, vol. 17, no. 5, pp. 395–404, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. J. D. Simon, D. Peles, K. Wakamatsu, and S. Ito, “Current challenges in understanding melanogenesis: bridging chemistry, biological control, morphology, and function,” Pigment Cell and Melanoma Research, vol. 22, no. 5, pp. 563–579, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. J.-Y. Yang, J.-H. Koo, Y.-G. Song et al., “Stimulation of melanogenesis by scoparone in B16 melanoma cells,” Acta Pharmacologica Sinica, vol. 27, no. 11, pp. 1467–1473, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. Y. J. Kim, J. K. No, J. S. Lee, M. S. Kim, and H. Y. Chung, “Antimelanogenic activity of 3,4-dihydroxyacetophenone: inhibition of tyrosinase and MITF,” Bioscience, Biotechnology and Biochemistry, vol. 70, no. 2, pp. 532–534, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Dörrie, V. Wellner, E. Kämpgen, G. Schuler, and N. Schaft, “An improved method for RNA isolation and removal of melanin contamination from melanoma tissue: implications for tumor antigen detection and amplification,” Journal of Immunological Methods, vol. 313, no. 1-2, pp. 119–128, 2006. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Nakajima, I. Shinoda, Y. Fukuwatari, and H. Hayasawa, “Arbutin increases the pigmentation of cultured human melanocytes through mechanisms other than the induction of tyrosinase activity,” Pigment Cell Research, vol. 11, no. 1, pp. 12–17, 1998. View at Google Scholar
  7. H. Ando, Y. Funasaka, M. Oka et al., “Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis,” Journal of Lipid Research, vol. 40, no. 7, pp. 1312–1316, 1999. View at Google Scholar · View at Scopus
  8. A. S. Peek and M. A. Behlke, “Design of active small interfering RNAs,” Current Opinion in Molecular Therapeutics, vol. 9, no. 2, pp. 110–118, 2007. View at Google Scholar · View at Scopus
  9. M. Ishikawa, I. Kawase, and F. Ishii, “Combination of amino acids reduces pigmentation in B16F0 melanoma cells,” Biological and Pharmaceutical Bulletin, vol. 30, no. 4, pp. 677–681, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. I. Bradáč, R. Svobodová Vařeková, M. Wacenovsky, M. Škrdla, M. Plchút, and M. Polčík, “siRNA selection criteria-Statistical analyses of applicability and significance,” Biochemical and Biophysical Research Communications, vol. 359, no. 1, pp. 83–87, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. A. Reynolds, D. Leake, Q. Boese, S. Scaringe, W. S. Marshall, and A. Khvorova, “Rational siRNA design for RNA interference,” Nature Biotechnology, vol. 22, no. 3, pp. 326–330, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. Y. Higashi, M. Asanuma, I. Miyazaki, and N. Ogawa, “Inhibition of tyrosinase reduces cell viability in catecholaminergic neuronal cells,” Journal of Neurochemistry, vol. 75, no. 4, pp. 1771–1774, 2000. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Boonanuntanasarn, G. Yoshizaki, K. Iwai, and T. Takeuchi, “Molecular cloning, gene expression in albino mutants and gene knockdown studies of tyrosinase mRNA in rainbow trout,” Pigment Cell Research, vol. 17, no. 4, pp. 413–421, 2004. View at Publisher · View at Google Scholar