Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli

Figure 1

Agarose gel 1% of the clone fragment of the gene PPE (1080 bp) in pQE-30 UA expression vector. Lane M1: DNA molecular weight marker 100 bp ladder. Lane1: PCR amplified PPE gene fragment of Mycobacterium avium subsp. paratuberculosis. Lane 2: Released insert of 1080 bp PPE gene fragment after digestion with NheI and EcoRI from recombinant plasmid pQE-30 UA PPE. Lane M1: Presented DNA molecular weight marker Lamda DNA/EcoRI/Hind III digest.