Expression of a Gene Encoding 34.9 kDa PPE Antigen of Mycobacterium avium subsp. paratuberculosis in E. coli

Figure 3

Western blot assay of the PPE protein expressed in E. coli cells. Lane M: Prestained protein molecular weight marker. Lane 1: Purified recombinant PPE-protein (37.1 kDa). Lane 2: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG induced). Lane 3: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG uninduced).