Table of Contents
Molecular Biology International
Volume 2011 (2011), Article ID 120176, 7 pages
Research Article

Allelic Diversity of Major Histocompatibility Complex Class II DRB Gene in Indian Cattle and Buffalo

1Animal Biotechnology Centre, National Dairy Research Institute, Karnal 1, Haryana 23001, India
2National Research Centre on Equines, Sirsa Road, Hisar 1, Haryana 25001, India
3KVK, SKUAST-Jammu, Bhaderwah, Jammu 182221, India

Received 20 September 2010; Accepted 6 January 2011

Academic Editor: Andrzej Kloczkowski

Copyright © 2011 Sachinandan De et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was conducted to study the diversity of MHC-DRB3 alleles in Indian cattle and buffalo breeds. Previously reported BoLA-DRB exon 2 alleles of Indian Zebu cattle, Bos taurus cattle, buffalo, sheep, and goats were analyzed for the identities and divergence among various allele sequences. Comparison of predicted amino acid residues of DRB3 exon 2 alleles with similar alleles from other ruminants revealed considerable congruence in amino acid substitution pattern. These alleles showed a high degree of nucleotide and amino acid polymorphism at positions forming peptide-binding regions. A higher rate of nonsynonymous substitution was detected at the peptide-binding regions, indicating that BoLA-DRB3 allelic sequence evolution was driven by positive selection.