Glucose-6-Phosphate Dehydrogenase of Trypanosomatids: Characterization, Target Validation, and Drug Discovery

Figure 2

G6PDH amino acid sequence alignment. The predicted amino acid sequences of T. cruzi G6PDH-long (DQ408239, sharing the highest identity with its T. brucei counterpart), T. brucei G6PDH-long (CAC07816), L. mexicana G6PDH (AAO37825), and Human G6PDH (AAL27011) were aligned using the CLUSTALW software. The first and second candidate initiator methionines in the T. cruzi and T. brucei sequences are indicated with arrows. The two regulatory cysteines of the T. cruzi G6PDH are boxed in yellow. Overlined, cofactor binding site; ^§G6PDH signature (residues belonging to the substrate binding site); ^#residues involved in substrate and cofactor binding.