Glucose-6-Phosphate Dehydrogenase of Trypanosomatids: Characterization, Target Validation, and Drug Discovery
Figure 3
T. cruzi G6PDH expression and specific activity are enhanced by oxidative stress in metacyclic trypomastigotes. Parasites were incubated for 4 to 6 h in the presence of 70 μM H2O2. Samples for enzymatic activity measurements and western blots were taken before (T0) and after the H2O2 addition. (a) G6PDH specific activity. The results are means ± SE of quadruplicates. (b) Autoradiography of a western blot corresponding to metacyclic trypomastigotes cell-free extracts (30 μg of total protein/lane). α-Tubulin was used as loading control. (Figure created with data from [43].)
