Research Article

5CAG and 5CTG Repeats Create Differential Impediment to the Progression of a Minimal Reconstituted T4 Replisome Depending on the Concentration of dNTPs

Figure 5

% ATP hydrolyzed by gp41 as a function of time in the presence of the minifork containing 17 repeats of 5′CTG and various proteins. The leading strand template of the minifork carried 17 repeats of 5′CTG and was included in all assays. The gp41 ATPase activity was measured as a function of time under various conditions: black filled circles and black curve (gp41 + minifork); blue filled circles and blue curve (gp41 + minifork + gp59); red filled circles and red curve (gp41 + minifork + gp43); green filled circles and green curve (gp41 + minifork + gp43 + gp59).
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