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Figure 2: Determination of long-chain fatty acyl-CoA ligase gene copy number. The nuclear DNA of L. donovani 2001 and 39 promastigotes was isolated and 16 μg was digested with different restriction enzymes. (A) Nuclear DNA digest stained with ethidium bromide (B) Southern blot of “A” with α-tubulin gene probe. (C) Southern blot of “A” with long-chain fatty acyl-CoA ligase probe. (D) PCR amplification of long-chain fatty acyl-CoA ligase gene (M: Marker, LCFA: 2010 bp of long-chain fatty acyl-CoA ligase gene).