Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle

Table 4

SNP detection in CD14 gene of CB cattle and the amino acid changes.

	Position of SNP	Synm/Nonsynm	Bos taurus	Bos taurus X Bos indicus	Type of mutation	Resulting amino acid change
1	A230C	Non-Synm	A	C	Transversion	N77T
2	G363C	Synm	G	C	Transversion
3	G412C	Non-synm	G	C	Transversion	A138P
4	G426A	Synm	G	A	Transition
5	A430C	Non-synm	A	C	Transversion	T144P
6	G440C	Non-synm	G	C	Transversion	S147T
7	C536T	Non-synm	C	T	Transition	A179V
8	C538G	Non-synm	C	G	Transversion	H180D
9	T556A	Non-synm	T	A	Transversion	C186S
10	A578G	Synm	A	G	Transition	E193G
11	T584C	Non-synm	T	C	Transition	L195P
12	C602T	Non-synm	C	T	Transition	S201F
13	G626C	Non-synm	G*	C	Transversion	S209T**
14	C627G	Non-synm	C*	G	Transversion	-do-
15	G628A	Non-synm	G	A	Transition	G210R
16	T635G	Non-synm	T	G	Transversion	M212R
17	T743A	Non-synm	T	A	Transversion	V248E
18	C778G	Non-synm	C	G	Transversion	L260V
19	C783G	Synm	C	G	Transversion
20	G790C	Non-synm	G	C	Transversion	A264L
21	C791T	Non-synm	C	T	Transition	A264L
22	G795C	Synm	G	C	Transversion
23	T798G	Synm	T	G	Transversion
24	G799T	Non-synm	G	T	Transversion	A267C
25	C800G	Non-synm	C	G	Transversion	A267C
26	C1058T	Non-synm	C	T	Transition	S353F
27	C1087T	Synm	C	T	Transition

*This nucleotide is present in cattle sequences gene bank Acc no. EU 148609, EU148610, EU148611, whereas cattle sequences NM174008, AF141313 contain the nucleotide sequence as in crossbred cattle. This SNP is already reported.
**Amino acid Serine is present in cattle sequences protein id Acc no. AAD32215, ABV68571, ABV68570, ABV68569, NP776433, ADB92696 (CB cattle).