A Perspective on the Emergence of Sialic Acids as Potent Determinants Affecting Leishmania Biology
Figure 5
Increased NO resistance and enhanced intracellular survival of 9-O-AcSAhigh promastigotes as compared to their de-O-acetylated forms. Untreated K27 (a), MON29 (b), and LV81 (c) with 9-O-AcSA (black square), esterase (white square), and sialidase (black triangle) treated promastigotes were exposed to NaNO2 and their viability (%) was estimated by MTT assay as described in [68]. Enhanced intracellular survival of promastigotes within macrophages (ϕ). Promastigotes of K27 (d) and LV81 (e) before (black square) and after de-O-acetylation (white square) were incubated with macrophages at a ratio of 1 : 10, for 0–96 h at 37°C and the interaction was quantified in terms of phagocytic index as described in details in [68] (reproduced and adapted from [68] with permission of the publishers and the Oxford University Press).
