Peptide Inhibition of Topoisomerase IB from Plasmodium falciparum
Figure 1
Effect of peptides on pfTopoI-mediated DNA relaxation. The effect of peptides on relaxation was assayed by incubating 200 fmol supercoiled plasmid with enzyme and peptide WRWYCRCK, KCCRCK, or WYCRCK at the following concentrations: 1.3 μM, 2.5 μM, 5 μM, 7.5 μM, 12.5 μM, 25 μM, or 50 μM. (a) Representative gel picture showing the relaxation activity of pfTopoI in the presence of increasing concentrations of peptide WRWYCRCK. (b) Representative gel picture showing the relaxation activity of pfTopoI in the presence of increasing concentrations of peptide KCCRCK. (c) Representative gel picture showing the relaxation activity of pfTopoI in the presence of increasing concentrations of peptide WYCRCK. C: negative control lane without any enzyme added; 0: positive control lane with pfTopoI but no peptide added; SC: supercoiled plasmid; Rn: relaxed topoisomers; Cat: supercoiled catenated plasmid.
