Establishment of Methylation-Specific PCR for the Mouse p53 Gene
MSP analysis. Primer sets used for amplification are designated as unmethylated (U) and methylated (M). Liver, kidney, and spleen samples were from two 8-week-old mice and two 122-week-old p53+/+ mice. Tumors were induced by a 90Sr-90Y beta ray in p53+/+ and p53+/− mice.