The SARAH Domain of RASSF1A and Its Tumor Suppressor Function
Figure 3
Effects of RASSF1AΔSARAH expression on mitosis. (a) HEK293 and COS7 cells were transfected with YFP-RASSF1A and YFP- RASSF1AΔSARAH (RASSF1AΔS) and stained using DAPI and anti-α-tubulin antibody. Yellow fluorescent is shown in green. (b) Rate of mitosis in HEK293 after transient expression. Cells with highly condensed chromosomes and spindle structures were classified as mitotic cells (c) Abnormal mitoses (monopolar, multipolar spindles and abnormal spindle fibers) were counted in HEK293 cells and the rate of abnormal mitosis is plotted. All experiments were done in triplicates and 500 cells each were evaluated. The mean and standard deviation were determined. Statistical significant -values are indicated.
