The SARAH Domain of RASSF1A and Its Tumor Suppressor Function

<table>Phosphorylation of MST1/2 is induced by staurosporine. A549 were treated with 3 <i>μ</i>M Staurosporine for 3 h. For transfections Turbofect (Fermentas) was used with 10 <i>μ</i>g of indicated constructs for 36 h. Total protein was isolated using Flag-lysis buffer, samples were denatured with Laemmli-buffer, separated in 10% SDS-PAGE and blotted onto PVDF membrane. First antibodies are: <i>α</i>-GAPDH, <i>α</i>-P-MST1 and <i>α</i>-Flag and secondary antibody are HRP coupled (sc2004 and sc2005 Santa cruz). ECL (WBKLS0100 Millipore) was used for detection with Versadoc (Biorad). Arrowheads indicate top down: P-MST, GST-Flag-RASSF1A, GST-Flag- RASSF1AΔSARAH (ΔS), Flag-RASSF1A, Flag-RASSF1AΔSARAH (ΔS), and GAPDH.</table>

Molecular Biology International

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Figure 5

Figure 5: The SARAH Domain of RASSF1A and Its Tumor Suppressor Function 