Table of Contents
Molecular Biology International
Volume 2012, Article ID 851670, 12 pages
Review Article

HIV Assembly and Budding: Ca2+ Signaling and Non-ESCRT Proteins Set the Stage

Department of Molecular Genetics & Microbiology, Stony Brook University, Life Sciences Building Room 248, Stony Brook, NY 11794-5222, USA

Received 31 January 2012; Accepted 26 March 2012

Academic Editor: Abdul A. Waheed

Copyright © 2012 Lorna S. Ehrlich and Carol A. Carter. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More than a decade has elapsed since the link between the endosomal sorting complex required for transport (ESCRT) machinery and HIV-1 protein trafficking and budding was first identified. L domains in HIV-1 Gag mediate recruitment of ESCRT which function in bud abscission releasing the viral particle from the host cell. Beyond virus budding, the ESCRT machinery is also involved in the endocytic pathway, cytokinesis, and autophagy. In the past few years, the number of non-ESCRT host proteins shown to be required in the assembly process has also grown. In this paper, we highlight the role of recently identified cellular factors that link ESCRT machinery to calcium signaling machinery and we suggest that this liaison contributes to setting the stage for productive ESCRT recruitment and mediation of abscission. Parallel paradigms for non-ESCRT roles in virus budding and cytokinesis will be discussed.