Molecular Biology International / 2014 / Article / Tab 3

Research Article

Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase

Table 3

Mutational spectra of six different PCR enzymes are presented. Results with Taq polymerase are mutations observed in sequencing clones from two independent PCR reactions. For indel mutations, the type of insertion or deletion is indicated.

Taq AccuPrime-Taq, HFKODPfu PhusionPwo

Transitions
 A•T→G•C67129366
 G•C→A•T2855554
Transversion
 A•T→T•A1111
 A•T→C•G2
 G•C→T•A3
Indels1
(T del.)
1
(T del.)
2
(A ins., TCT del in (TCT)5 run)

Insertion = ins.; deletion = del.