Figure 4: RAPD analysis of soil DNA samples isolated by five methods using random decameric primers. (a) OPA 3 and (b) OPA 13. Lane M: DNA EcoRI/Hind III double digest marker; lane 1: Soil Master DNA extraction kit; lane 2: mannitol-PBS-CTAB method; lane 3: mannitol-PBS-PEG/NaCl method; lane 4: mannitol-PBS-PCI method; lane 5: PEG/NaCl method without liquid nitrogen.